Leverage the power of 454 Sequencing technology on your desktop

With the GS Junior System, you don’t have to be an IT or bioinformatics expert to dig down to the biology and accomplish your research goals. All 454 data processing can be performed on the accompanying computer, pre-installed with GUI-based GS Data Analysis Software. There is no need for command-line instructions or a costly high-end computing cluster.

The GS Data Analysis Software package includes easy-to-use tools for assembly, mapping and variant detection.

GS De Novo Assembler

Point-and-click genome and transcriptome assembly

• Produce high-quality assemblies for microbial genomes in as little as 15 minutes and larger sized genomes in hours
• Use single reads alone or combine with paired end reads to improve assemblies by ordering and joining contigs into scaffolds
• Perform de novo assembly of EST reads from cDNA library sequencing runs—the software is designed to handle the specific needs of transcriptome assembly and analysis including splice variant detection and transcript isoform identification
• Straightforward graphical software environment or command line operation
• Data output: fna.file (sequence of contigs, FASTA format), qual.file (corresponding Phred equivalent quality score), ace.file (alignment of the reads to contig sequence), optional consed output and more

GS Reference Mapper

Automated variation detection for resequencing projects

• Map reads to any reference genome and generate a consensus sequence
• Quickly identify high confidence differences compared to the reference genome, which are singled out in a separate file
• Explore the full spectrum of genomic variation
• Local variation detection: SNPs, insertions and deletion (blocks up to 50 bases)
• Structural variation detection: rearrangement points and regions
• Data outputs: fna.file (sequence of contigs, FASTA format), qual.file (corresponding Phred equivalent quality score), ace.file (consensus alignment of the reads against a given reference sequence)

GS Amplicon Variant Analyzer

High-sensitivity variation detection from amplicon-based samples

• Automatically aligns amplicon reads, with or without a reference sequence
• Identifies variants and reports frequencies in large mixed sample pools
• Screen against known variants (with reference) and discover novel variants
• Perform haplotyping/phasing – identify multiple variants over the full amplicon length
• Detect low-frequency (<1%) variants
• Variant GUI view shows alignments, position differences and % frequency graphically with color coding
• Flexible project construction: separate samples / results based on MID tags (“barcodes”), directly import assets into project, use filtered / modified input data files
• Data outputs: ace.file (alignment of the reads against a reference sequence), png.file (graphical file format, tab delimited text file)