The GS Junior System is an optimized solution for a wide variety of sequencing applications.

De Novo Sequencing

Affordable sequencing of whole microbial genomes

Sequence complete bacterial, viral, small fungal and other small genomes or multiple BACs and plasmids on the GS Junior System. Use long single reads alone, or in combination with paired end reads to generate high-quality de novo assemblies with few contigs. Perform comparative analysis of multiple whole genomes and identify differences between samples. Dedicated point-and-click software saves time and labor by generating high-quality assemblies in as little as 15 minutes. The GS Junior System offers a whole genome sequencing solution for biologist and bioinformaticians alike!

Targeted Resequencing

High sensitivity amplicon sequencing

Perform ultra-deep sequencing of amplicons (PCR products) with the GS Junior System. Generating an average of 100,000 high quality reads per run, the system is ideally suited for detecting low level variants (<1% frequency) in pooled amplicon samples, such as viral or human DNA. For many targeted sequencing projects, dozens or hundreds of amplicons can be tiled across genomic regions of interest (i.e. disease-associated regions) and sequenced together.

DNA sequences, called Multiplex Identifiers (MIDs) or “barcodes”, can be added to samples to specifically tag them in a GS Junior run. Each MID is recognized and separated by the data analysis software after the sequencing run, providing high confidence in assigning an individual sequencing read to the correct sample.

For high-throughput amplicon sequencing projects, the Fluidigm Access Array System offers an automated sample preparation workflow, generating up to 2,304 unique amplicons which are ready for emPCR and sequencing with the GS Junior System. Learn more.

Targeted sequencing using sample-enrichment technologies

Sequence capture technologies allow researchers to target and sequence only the regions of interest in the genome and assess the genetic variation within the captured DNA regions. The GS Junior System is ideal for small sequence capture projects that require sequencing of 40 million bases or less.

Metagenomic Characterization

Get the complete picture of species diversity

The GS Junior System enables a comprehensive view into the diversity and metabolic profile of an complex environmental samples. Analysis of 100,000 reads can yield a high percentage of species identification in complex metagenomes and even higher in less complex samples. The long reads provide the enormous specificity needed to compare sequenced reads against DNA or protein databases and give unambiguous assignment of even closely related species.

And more…

The GS Junior System offers straightforward protocols for shotgun and paired end (3 Kb, 8 Kb, 20 Kb) sequencing, as well as cDNA protocols for comprehensive transcriptome analysis. The proven 454 Sequencing technology has enabled hundreds of peer-reviewed publications in a wide variety or applications. Click here to see the complete list.